Janina Whitening Mouth Rinse
HIGH RESOLUTION NMR INVESTIGATIONS OF THE OXIDATIVE
CONSUMPTION OF SALIVARY BIOMOLECULES BY A TOOTH-WHITENING MOUTHRINSE
Martin Grootveld1*,Edward Lynch2, Andrew W.D. Claxson1,
Barry P. Mills1 and Christopher J.L. Silwood1
1Inflammation Research Group and 2Department of Conservative Dentistry, St. Bartholomew’s
and the Royal London School of Medicine and Dentistry
*Corresponding author.
Telephone : +44 171 377 7000 ext. 2945
Fax : +44 171 377 7763
E-mail : c.j.silwood@mds.qmw.ac.uk
ABSTRACT
A multicomponent evaluation of the oxidative consumption of salivary biomolecules
by a tooth-whitening mouthrinse preparation has been performed using high resolution
proton nuclear magnetic resonance spectroscopy. Unstimulated human saliva samples
(n = 10) were treated with aliquots of a solution derived from the above product
and 600 MHz 1H NMR spectra acquired on these samples demonstrated that hydrogen
peroxide (H2O2) and/or peroxodisulphate (S2O82-) effected the oxidative decarboxylation
of the salivary electron-donor pyruvate (to acetate and CO2). Experiments conducted
on chemical model systems confirmed the consumption of pyruvate by this product,
and also revealed that the amino acids cysteine and methionine (precursors to
volatile sulphur compounds responsible for oral malodour) were oxidatively transformed
to cystine and methionine sulphoxide respectively. In conclusion, high field proton
NMR analysis provides much valuable molecular information regarding the fate of
tooth-whitening oxidants in human saliva and permits an assessment of the mechanisms
of action of oral health care products containing these agents. The biochemical,
periodontal and potential therapeutic significance of the results obtained are
discussed.
Home | Contact
| Archive | Forum
| Opale | Diamond
| Liquid | Rinse
| Why Janina | Media
| Clinical | Buy Online
| Other Outlets | FAQs
| Sitemap